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For the designed 12-week exposure experiments, rats were restrained in individual tubes that were plugged into an exposure manifold with just the snout exposed to the exposure atmosphere, which served to minimize dermal exposure

As for e-cigs, although there is no smoke produced, the aerosol spray contents are spread to the bystanders in the nearby capacity of people using e-cigs, suggesting that some second hand exposure does exist

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DATA AVAILABILITY The data supporting this research can be found in the Supplementary file