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Thus, diagnosis of dBTHAL can be challenging since it requires a high index of suspicion and the diagnosis may be delayed for years hampering the access to timely treatment interventions

Recent studies found that transcription factors such as p53, the tumor suppressor BAP1 (BRCA1-associated protein 1), and ATF3 (activating transcription factor 3) increase cellular susceptibility to ferroptosis via decreased cystine uptake by inhibiting SLC7A11 expression [50,51,52]

PMID: 28931444

In this purification, a glutathione is combined with the arm of the gel through a sulfur bond, and then the specific action force between glutathione and glutathione S-transferase (i.e., GST-tag) is used to make the GST-tagged fusion protein combine with the arm glutathione on the gel, thereby separating the labeled protein from other proteins